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TaKaRa fragmentation kit
Fragmentation Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 107 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ribogone+mammalian/RiboGone+-+Mammalian/10__1016_slash_j__celld__2026__100015-128-17-19
Average 95 stars, based on 107 article reviews

fragmentation kit - by Bioz Stars, 2026-09

95/100 stars

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RNA Extraction:

Article Title: Type 2 diabetes susceptibility gene GRK5 regulates physiological pancreatic β-cell proliferation via phosphorylation of HDAC5.
Article Snippet: RNA was isolated with TRIzol, DNase treated (Turbo DNAse Free; Thermo Fisher Scientific), and reverse transcribed with Superscript III (Thermo Fisher Scientific) as previously described.3 TaqMan quantitative PCR (qPCR) was performed as described.3 Primers used were: mouse Grk5 (forward [F], 50-GCACTCAAC GAAAAGCAGATTC-30; reverse [R], 50-GTGCATCTTTGGTTTCATAGGC-30; and probe 50-AGGTCAA CAGCCAGTTTGTGGTCA-30), mouse Cdkn1a (F, 50-CTGAGCGGCCTGAAGATT-30; R, 50-ATCTGCGCTT GGAGTGATAG-30; and probe 50-AAATCTGTCAGGCTGGTCTGCCTC-30), mouse Gusb (F, 50-TCTAGCTG GAAATGTTCACTGCCCTG-30; R, 50-CACCCCTACCACTTACATCG-30; and probe 50-ACTTTGCCACCCT CATCC-30), human GRK5 (F, 50-AGGTCTTCACACTGGCTAATG-30; R, 50-CAATGGAGCTGGAAAACAT CG-30; and probe 50-AGGAAAGCGCAAAGGGAAAAGCAAG-30), and human TBP (F, 50-TGGGATTA TATTCGGCGTTTCGGGC-30; R, 50-GAGAGTTCTGGGATTGTACCG-30; and probe 50-ATCCTCATGAT TACCGCAGC-30). .. Following total RNA extraction and DNase treatment, samples were rRNA depleted utilizing the RiboGone Mammalian-Low Input Ribosomal RNA Removal Kit (Takara Bio USA Inc., Mountain View, CA, USA), and Agencourt AMPure XP SPRI beads (Beckman Coulter). rRNA depleted total RNA was assessed for rRNA depletion, RNA integrity, size distribution, and concentration prior to library generation with the Agilent RNA Pico 6000 kit on the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). rRNA depleted RNA passing quality control was used in the SMARTer Stranded RNA-Seq Kit (Takara Bio USA). ..

Article Title: Type 2 diabetes susceptibility gene GRK5 regulates physiological pancreatic β-cell proliferation via phosphorylation of HDAC5 in mice
Article Snippet: .. Following total RNA extraction and DNase treatment, samples were rRNA depleted utilizing the RiboGone Mammalian-Low Input Ribosomal RNA Removal Kit (Takara Bio USA Inc., Mountain View, CA, USA), and Agencourt AMPure XP SPRI beads (Beckman Coulter). rRNA depleted total RNA was assessed for rRNA depletion, RNA integrity, size distribution, and concentration prior to library generation with the Agilent RNA Pico 6000 kit on the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). rRNA depleted RNA passing quality control was used in the SMARTer Stranded RNA-Seq Kit (Takara Bio USA). ..

Concentration Assay:

Control:

RNA sequencing:

Article Title: High-throughput proteo-genomic identification of functional endothelial cell microproteins encoded by non-canonical small open reading frames
Article Snippet: RNA and library preparation integrity were verified on a LabChip Gx Touch 24 Nucleic 567 Acid Analyzer (Perkin Elmer). .. Total RNA (0.5-1 μg) was used as input for ribosomal depletion 568 with RiboGone-Mammalian (Takara Bio, Gennevilliers, France) followed by library preparation 569 using the SMARTer stranded total RNA sample prep kit (Takara Bio, Gennevilliers, France) 570 or Corall Total RNA-Seq Library Prep Kit (Lexogen), following manufacturer’s instructions. .. 515 AAV-PCSK9 generation and partial carotid artery ligation 516 An AAV serotype 8 vector for expression of the murine D377Y-PCSK9 cDNA (AAV-PCSK9) 517 was produced using the two-plasmid-method by co-transfecting AAV/D377Y-mPCSK9 (gift 518 from Jacob Bentzon; Addgene plasmid # 58376)52 together with the helper plasmid pDP853 in 519 HEK-293T cells using polyethylenimine (Sigma-Aldrich).

Sample Prep:

Article Title: Spurious transcription causing innate immune responses is prevented by 5-hydroxymethylcytosine.
Article Snippet: RNA and library preparation integrity were verified with BioAnalyzer 2100 (Agilent) or LabChip Gx Touch 24 (Perkin Elmer). .. Total RNA (50 ng) was used as input for ribosomal depletion with RiboGone-Mammalian (Clontech) followed by library preparation using SMARTer Stranded Total RNA Sample Prep Kit (Clontech). .. Sequencing was performed on the NextSeq500 instrument (Illumina) using v.2 chemistry, resulting in average of 44 M reads per library with 1 × 75bp single-end setup.

Article Title: YAP and TAZ protect against white adipocyte cell death during obesity
Article Snippet: For RNA-seq, RNA and library preparation integrity were verified with a BioAnalyzer 2100 (Agilent) or LabChip Gx Touch 24 (Perkin Elmer). .. Twenty nanograms of total RNA was used as starting material for ribosomal depletion with RiboGone-Mammalian (Clontech) followed by library preparation using SMARTer Stranded Total RNA Sample Prep Kit (Clontech). .. Sequencing was performed on the NextSeq500 instrument (Illumina) using v2 chemistry, resulting in an average of 35 M reads per library with 1 × 75 bp single end setup.

Article Title: Spurious transcription causing innate immune responses is prevented by 5-hydroxymethylcytosine
Article Snippet: RNA and library preparation integrity were verified with BioAnalyzer 2100 (Agilent) or LabChip Gx Touch 24 (Perkin Elmer). .. Total RNA (50 ng) was used as input for ribosomal depletion with RiboGone-Mammalian (Clontech) followed by library preparation using SMARTer Stranded Total RNA Sample Prep Kit (Clontech). .. Sequencing was performed on the NextSeq500 instrument (Illumina) using v.2 chemistry, resulting in average of 44 M reads per library with 1 × 75bp single-end setup.

other:

Article Title: Transcriptional coactivator MED15 is required for beta cell maturation
Article Snippet: Following total RNA extraction and DNase treatment , samples were rRNA depleted using a RiboGoneTM Mammalian-Low Input Ribosomal RNA Removal Kit (Takara Bio USA 634847), Agencourt AMPure XP SPRI beads (Beckman Coulter A63881), and DynaMag-96 side magnet (ThermoFisher Scientific 12331D). rRNA-depleted RNA samples were assessed for rRNA depletion, RNA integrity, size distribution, and concentration prior to library generation with the Agilent RNA Pico 6000 kit on an Agilent 2100 Bioanalyzer (Agilent Technologies 5067-1511). rRNA-depleted RNA passing quality control was used in the SMARTer Stranded RNA-seq Kit (Takara Bio USA 634839).

Isolation:

Article Title: Dual transcriptional analysis reveals adaptation of host and pathogen to intracellular survival of Pseudomonas aeruginosa associated with urinary tract infection
Article Snippet: .. Samples were lysed by incubating at 75°C for 5 minutes and RNA was isolated using SPRI paramagnetic beads. rRNA was depleted using RiboGone-Mammalian (Clontech Laboratories, Inc), cDNA was synthesized using the SMARTer Universal Low Input RNA Kit (Clontech Laboratories, Inc) and library preparation was performed using the Low Input Library Prep Kit v2 (Clontech Laboratories, Inc). ..

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